Featured Blog: Cryopreservation of Equine Embryos

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January 30, 2015

Posted by Dr. Dave Scofield in Mare Management

Embryo vitrification doesn't sound much like "freezing embryos,” but the end product certainly is a cryopreserved equine embryo, frozen in liquid nitrogen for the preservation of genetics and transfer into a recipient mare at a later time. For the sake of some readers of this blog, an embryo is the result of fertilizing an oocyte (egg) with sperm and allowing the initial stages of development to occur. Embryo vitrification is the process whereby we freeze equine embryos for storage for indefinite periods of time prior to transfer into a recipient mare. We have the technology to cryopreserve equine sperm and equine embryos, but not equine oocytes. Herein lays the answer to the most common question that comes up in conversation regarding this blog topic. Some readers assume that since we can freeze the male generated sperm, we can likewise freeze the female generated oocyte. Unfortunately, due to some very sensitive cytoskeletal components in the oocyte, the technology does not exist to freeze equine oocytes. As we proceed, you will understand the process of embryo vitrification and we will delve into areas of research that are improving the success rates of vitrified embryos in generating live foals.

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Ovum Pickup in the Mare

January 03, 2015

Posted by Dr. Gabe Young in Mare Management

Breeding horses has come a long ways in the last 50 years. Veterinarians have been able to overcome many of the obstacles presented when breeding horses with advancements in artificial insemination and embryo transfer. However, there are still times when infertility of either the mare, stallion or both prevent getting foals on the ground. The most recent advances in assisted reproductive technologies, Ovum Pickup and Intra-Cytoplasmic Sperm Injection (ICSI), have allowed us at Weatherford Equine Breeding Center (WEBC) to take the next step in overcoming these obstacles with some promising results.

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Effect of Number and Timing of Equine Frozen Semen Inseminations on Fertility

December 03, 2014

Posted by Dr. Ed Squires in Mare Management

Further expansion in the use of frozen semen is dependent upon developing simplified strategies for insemination. SBS has developed a timed insemination protocol where mares are only examined once per day during estrus and inseminated at 24 and 40 hr after hCG or 30 and 40 hr after GnRH. This approach allows frozen semen mares to be managed similar to those bred with cooled shipped semen. A couple of arguments against this approach with frozen semen is that it takes too much semen when mares are bred twice in one cycle and that if the mare is inseminated more than once per cycle the fertility will be lowered because of post-breeding induced endometritis. However, in a new retrospective study across 1871 mare cycles bred by SBS affiliate labs, we report additional support for the use of two inseminations per breeding cycle.

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Quality Control is at the Core of the SBS Difference

October 31, 2014

Posted by Paul Loomis in Frozen Semen

Quality Control_SBS Extender LabThe SBS network of affiliates is the acknowledged leader in the field of cryopreservation of equine semen. Each year we freeze thousands of ejaculates equating to tens of thousands of doses. In fact, this year the SBS Network will surpass the 50,000 ejaculate milestone since the company’s founding in 1987. When SBS was founded the company leadership made a commitment to quality of the finished product above all else. We wanted to put technology to use to help grow the equine frozen semen AI industry in the most responsible manner possible. It was clear early on that one of the major factors limiting the application of frozen semen in the horse industry was inconsistent quality of the product being put on the market. Breeders and veterinarians experienced frustration with attempts to use expensive semen that in many cases was of very poor quality. We knew that in order for breeders to realize the full potential of frozen semen technology they would have to be convinced that by applying strict standards for quality, frozen semen could be produced to achieve results similar to cooled semen. We also knew that these results would have to be produced in an efficient and economical way. Quality became the cornerstone upon which we would build our business.

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Please Note - photos used in these news articles are available in the public domain, have been purchased through istockphoto or (when referencing breeders or horses) have been submitted to Select Breeders Services Inc. by the breeding farm or horse owner. Photo credit has been provided where applicable. If at anytime you see something that needs to be addressed please feel free to contact us directly.

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